Mary Queen of Scots expresses deep emotional longing and regret in her poem. She reflects on the constant presence of her absent love, which haunts her during all moments of the day and night, whether she is surrounded by nature or in solitude. This sense of regret is a profound feeling that permeates her thoughts and experiences.

As she prepares for sleep, the memories of her beloved become more vivid, emphasizing a sense of loneliness. The physical absence of her loved one intensifies her feelings, and she longs for their touch, showcasing the depth of her emotional struggle and the powerful impact of love and loss in her life.